Akademik Veri Yönetim Sistemi
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler, cilt.8, sa.2, ss.293-302, 2020 (Hakemli Dergi)
Polycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicindesigned and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinningmethod with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli.The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed theaddition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cellshowed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interactionamong gentamicin, plasmid DNA and electrospun fiber matrix.